51�Թ���

Lost your keys? Can’t remember where you parked the car? If only you had the memory of a mountain chickadee.��

These half-ounce birds, with brains slightly larger than a pea, stash tens of thousands of food items like seeds in tree bark, under dead leaves and inside pinecones across the mountains. When winter arrives, they can recall the exact locations of their caches, a skill that helps them survive the bitter cold and deep snow.��

In a published April 17 in the journal Current Biology, researchers at 51�Թ��� and the University of Nevada, Reno identify nearly a hundred genes associated with the birds’ spatial memory, or ability to recall the locations of objects. The paper also suggests a potential trade-off may exist between having a solid long-term memory and being able to quickly ditch old memories to form new ones.

The findings could help biologists better understand the evolution of spatial memory in animals, including humans,��said Georgy Semenov, the paper’s lead author and a research associate in the Department of Ecology and Evolutionary Biology. “What we observed also provides valuable insight into how species may adapt to rapidly fluctuating conditions, especially in the context of climate change.”

“Chickadees are impressive birds,” said Scott Taylor, the director of 51�Թ���’s Mountain Research Station and associate professor in the Department of Ecology and Evolutionary Biology. “Their spatial memory is much more developed than many other birds that don't have to have this strategy to survive cold winters.”

To evaluate the spatial memory of wild mountain chickadees, Taylor’s collaborators at the University of Nevada, Reno, led by biologist professor, Vladimir Pravosudov, designed a clever test. They hung multiple feeder arrays, each with eight bird feeders with seeds in California’s Sierra Nevada mountains. Each feeder has a gate with a radio frequency reader that can detect a tag researchers put on chickadees. The team then programmed each gate to only open to certain birds, so that the chickadees had to remember the location of the feeders that would open to them.��

Top: A mountain chickadee eats seeds from a feeder.��(Credit: Nicholas Goda) Bottom: The feeder array used to test chickadees'��spatial memory. (Credi: Yvaine Ye)

Pravosudov and his team then counted how many times each chickadee landed on the wrong feeders before they recalled the right one. The theory is that birds with better spatial memory would have a lower error rate.��

Using blood samples, the team at 51�Թ��� also sequenced the entire genome of 162 tagged chickadees, creating the largest dataset ever collected for evaluating the genetic basis of chickadee cognitive ability. By comparing the birds’ genomes with their performance on the feeder test, the team identified 97 genes associated with chickadees’ spatial learning and memory. Birds with specific genetic variants at these genes made fewer wrong attempts before landing on their designated feeders.��

A large proportion of these variants are associated with neuron formation in the hippocampus, a part of the brain that’s responsible for learning and memory, according to paper co-author Sara Padula, a doctoral student in the Department of Ecology and Evolutionary Biology. Padula��conducted a literature review on chickadees' spatial memory��for the paper along with Ajay Patel, a doctoral��student��in the Taylor Lab.��

"We found in previous literature that many of these��genetic variants��in chickadees are associated with behavioral disorders in other animals. So this finding can help us better understand��animal behaviors in general,"��Semenov said.

Understanding what genes are associated with the bird's spatial memory��can help biologists track how the��trait evolves, according to Taylor. He��adds��that the common ancestor of all North American chickadees cached food. But of the seven species of chickadees now found here, two do not.��“They live in a milder environment where food is generally available year-round. Now that we know the gene regions that underlie spatial memory, we can look at what variation looks like in these species that have lost caching,” he said. ��

A trade-off

Chickadees that have exceptional spatial memory can live up to nine years, which is a long time for a small bird. But the study suggests that having good long-term memory may come at a price.��

After running the initial task for a few days, Pravosudov’s team assigned new feeders to the birds.��

To the team’s surprise, chickadees that performed better in the initial test tended to struggle with switching to the new feeder. They seemed to have a harder time abandoning their initial memories and creating new ones.

“In a more variable environment, what our collaborators found suggests that chickadees with good long-term memory may have a disadvantage. For example, if there is an unexpected snowstorm, these birds may keep trying to visit caches that have been buried in the snow, rather than forgetting them and looking for other caches,” Padula said.��

A shifting climate

Facing a rapidly changing climate, birds that can quickly form new memories may survive better.

“We hope to track how chickadee's spatial memory��evolves under pressure from climate change. The good news is��that these birds��appear to have genetic variations in the population that could potentially allow them to adapt. Species lacking such variations may not be able to��do that," Semenov said.

This winter, Semenov, Taylor��and the team set up the same type of feeder array at the university’s Mountain Research Station west of 51�Թ���.��

For the last one million years, the mountain chickadees in the Rocky Mountains have evolved independently from those in the Sierra Nevada Mountains. The team hopes to investigate whether the two groups of birds have evolved spatial memory in the same way across different geographic regions.��

The team is also interested in learning if black-capped chickadees, which coexist with mountain chickadees in the Rocky Mountains, exhibit different spatial memory skills. They’ll continue the feeder experiment at the Mountain Research Station during the upcoming winters to collect more data.

“We don't have to travel to a remote part of the world like Antarctica to study how animals might respond to climate change. We can do it with these birds that most North Americans are familiar with. I think that’s something special about chickadees,” Taylor said.